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psnapf h2b sequence  (Addgene inc)


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    Structured Review

    Addgene inc psnapf h2b sequence
    Psnapf H2b Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psnapf+h2b+control+plasmid/pSNAPf-H2B+Control+Plasmid+(Plasmid+%23101124)/bio_rxiv__2025__06__06__658161-220-1-7
    Average 93 stars, based on 6 article reviews
    psnapf h2b sequence - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging
    Article Snippet: .. DNA encoding SNAPf or H2B-SNAPf was amplified from pSNAPf-H2B control plasmid (Addgene no. 101124) to generate an insert. .. To generate a backbone containing an organelle-specific targeting group and a fluorescent protein, the plasmids pmTurquoise2-ER (Addgene no. 36204) and pmTurquoise2-Golgi (Addgene no. 36205) were used.

    Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging
    Article Snippet: .. DNA encoding SNAPf or H2B-SNAPf was amplified from pSNAPf-H2B control plasmid (Addgene #101124) to generate an insert. .. To generate a backbone containing an organelle-specific targeting group and a fluorescent protein, the plasmids pmTurquoise2-ER (Addgene #36204) and pmTurquoise2-Golgi (Addgene #36205) were used.

    Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging.
    Article Snippet: .. DNA encoding SNAPf or H2B-SNAPf was amplified from pSNAPf-H2B control plasmid (Addgene no. 101124) to generate an insert. .. To generate a backbone containing an organelle-specific targeting group and a fluorescent protein, the plasmids pmTurquoise2-ER (Addgene no. 36204) and pmTurquoise2-Golgi (Addgene no. 36205) were used.

    Control:

    Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging
    Article Snippet: .. DNA encoding SNAPf or H2B-SNAPf was amplified from pSNAPf-H2B control plasmid (Addgene no. 101124) to generate an insert. .. To generate a backbone containing an organelle-specific targeting group and a fluorescent protein, the plasmids pmTurquoise2-ER (Addgene no. 36204) and pmTurquoise2-Golgi (Addgene no. 36205) were used.

    Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging
    Article Snippet: .. DNA encoding SNAPf or H2B-SNAPf was amplified from pSNAPf-H2B control plasmid (Addgene #101124) to generate an insert. .. To generate a backbone containing an organelle-specific targeting group and a fluorescent protein, the plasmids pmTurquoise2-ER (Addgene #36204) and pmTurquoise2-Golgi (Addgene #36205) were used.

    Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging.
    Article Snippet: .. DNA encoding SNAPf or H2B-SNAPf was amplified from pSNAPf-H2B control plasmid (Addgene no. 101124) to generate an insert. .. To generate a backbone containing an organelle-specific targeting group and a fluorescent protein, the plasmids pmTurquoise2-ER (Addgene no. 36204) and pmTurquoise2-Golgi (Addgene no. 36205) were used.

    Article Title: A Microtubule Mechanostat Enables Cells to Navigate Confined Environments
    Article Snippet: .. Entry vectors were created as follows. pENTR-SNAPf-H2B was created by restriction digest of pENTR1a-GFP-N2 (FR1) (Addgene, #19364 Eric Campeau and Paul Kaufman) and pSNAPf-H2B control plasmid (Addgene #101124, New England Biolabs and Ana Egana) using KpnI and NotI, and ligated together using T4 DNA ligase (New England Biolabs, M0202). .. The following constructs were created using HiFi assembly (New England Biolabs, E2521L) Compatible fragments were combined as per manufacturer’s instructions. pENTR-3xNLS-mScarlet-I-nucleoplasmin-NLS was created using HiFi assembly (New England Biolabs).

    Article Title: Silicon functionalization expands the repertoire of Si-rhodamine fluorescent probes
    Article Snippet: .. Imaging of SNAP-tag-expressing cells HeLa cells were seeded in 35 mm glass bottom dishes (Cellvis, catalog no. D35- 28-0-N) and transfected with pSNAPf-H2B control plasmid (Addgene #101124). .. Transient transfections were performed using Lipofectamine 2000 (Invitrogen, catalog no.1168019) following the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging
    Article Snippet: .. DNA encoding SNAPf or H2B-SNAPf was amplified from pSNAPf-H2B control plasmid (Addgene no. 101124) to generate an insert. .. To generate a backbone containing an organelle-specific targeting group and a fluorescent protein, the plasmids pmTurquoise2-ER (Addgene no. 36204) and pmTurquoise2-Golgi (Addgene no. 36205) were used.

    Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging
    Article Snippet: .. DNA encoding SNAPf or H2B-SNAPf was amplified from pSNAPf-H2B control plasmid (Addgene #101124) to generate an insert. .. To generate a backbone containing an organelle-specific targeting group and a fluorescent protein, the plasmids pmTurquoise2-ER (Addgene #36204) and pmTurquoise2-Golgi (Addgene #36205) were used.

    Article Title: A general strategy to develop fluorogenic polymethine dyes for bioimaging.
    Article Snippet: .. DNA encoding SNAPf or H2B-SNAPf was amplified from pSNAPf-H2B control plasmid (Addgene no. 101124) to generate an insert. .. To generate a backbone containing an organelle-specific targeting group and a fluorescent protein, the plasmids pmTurquoise2-ER (Addgene no. 36204) and pmTurquoise2-Golgi (Addgene no. 36205) were used.

    Article Title: A Microtubule Mechanostat Enables Cells to Navigate Confined Environments
    Article Snippet: .. Entry vectors were created as follows. pENTR-SNAPf-H2B was created by restriction digest of pENTR1a-GFP-N2 (FR1) (Addgene, #19364 Eric Campeau and Paul Kaufman) and pSNAPf-H2B control plasmid (Addgene #101124, New England Biolabs and Ana Egana) using KpnI and NotI, and ligated together using T4 DNA ligase (New England Biolabs, M0202). .. The following constructs were created using HiFi assembly (New England Biolabs, E2521L) Compatible fragments were combined as per manufacturer’s instructions. pENTR-3xNLS-mScarlet-I-nucleoplasmin-NLS was created using HiFi assembly (New England Biolabs).

    Article Title: Silicon functionalization expands the repertoire of Si-rhodamine fluorescent probes
    Article Snippet: .. Imaging of SNAP-tag-expressing cells HeLa cells were seeded in 35 mm glass bottom dishes (Cellvis, catalog no. D35- 28-0-N) and transfected with pSNAPf-H2B control plasmid (Addgene #101124). .. Transient transfections were performed using Lipofectamine 2000 (Invitrogen, catalog no.1168019) following the manufacturer’s instructions.

    Imaging:

    Article Title: Silicon functionalization expands the repertoire of Si-rhodamine fluorescent probes
    Article Snippet: .. Imaging of SNAP-tag-expressing cells HeLa cells were seeded in 35 mm glass bottom dishes (Cellvis, catalog no. D35- 28-0-N) and transfected with pSNAPf-H2B control plasmid (Addgene #101124). .. Transient transfections were performed using Lipofectamine 2000 (Invitrogen, catalog no.1168019) following the manufacturer’s instructions.

    Transfection:

    Article Title: Silicon functionalization expands the repertoire of Si-rhodamine fluorescent probes
    Article Snippet: .. Imaging of SNAP-tag-expressing cells HeLa cells were seeded in 35 mm glass bottom dishes (Cellvis, catalog no. D35- 28-0-N) and transfected with pSNAPf-H2B control plasmid (Addgene #101124). .. Transient transfections were performed using Lipofectamine 2000 (Invitrogen, catalog no.1168019) following the manufacturer’s instructions.



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